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Cephalopod ink is a dark-coloured or luminous released into water by most species of , usually as an escape mechanism. All cephalopods, with the exception of the and the (deep-sea octopuses),Hanlon, Roger T. and Messenger, John B. (1999) Cephalopod Behaviour, p. 2. Cambridge University Press. are able to release ink to confuse .

(2025). 9780632060481, Blackwell. .

The ink is released from the (located between the ) and is dispersed more widely when its release is accompanied by a jet of water from the siphon. Its dark colour is caused by its main constituent, . Each species of cephalopod produces slightly differently coloured inks; generally, octopuses produce black ink, squid ink is blue-black, and cuttlefish ink is a shade of brown.

A number of other aquatic molluscs have similar responses to attack, including the clade known as .


Properties and chemistry
Cephalopod ink contains a number of chemicals in a variety of different concentrations, depending on the species. However, its main constituents are and . It can also contain, among others, , , and , as well as small amounts of free , including , , , , and .


Attack protection
Inking has been shown to protect species of squids against predatory fish attacks, as well during the capture phase as during the consummatory phase, due to visual and chemical effects of the ink releases.


Chemical effects
Many cephalopod predators (for instance ) have advanced systems, and some anecdotal evidenceMacGinitie, G.E. and MacGinitie, N. (1968) Natural History of Marine Animals, pp. 395–397, 2nd ed. McGraw-Hill, New York. suggests that compounds (such as ) found in cephalopod ink can irritate, numb or even deactivate such apparatus. Few controlled experiments have been conducted to substantiate this. Cephalopod ink is nonetheless generally thought to be more sophisticated than a simple "smoke screen"; the ink of a number of squid and cuttlefish has been shown to function as a conspecific . Computer simulations have suggested the melanin deployed by cuttlefish may be particularly effective against the scent receptors of , overwhelming the fishes' narrow but extremely intense scent range to deter them from predation.Prillaman, McKenzie. "Cuttlefish ink may overwhelm sharks’ sense of smell", Science News. 5 February 2025.


Physical properties
Sepia officinalis ink forms a suspension composed by spheric particles with a size between 80 and 150 nm (measured by TRPS and SEM). The particles have a density of , which may be due to the amount of metals that the ink has in its composition (4.7% in weight).

Heteroteuthis dispar is a cephalopod species known for releasing luminous ink. The light comes from a substance produced by a dedicated organ before being transferred into the ink sac.


Types of ink shapes
The shapes taken by ink releases are classified as six types:

  • pseudomorphs;
  • pseudomorph series;
  • ink ropes;
  • clouds/smokescreens;
  • diffuse puffs;
  • mantle fills.


Inking behaviours

Escape strategies
Two distinct behaviors have been observed in inking cephalopods. The first is the release of large amounts of ink into the water by the cephalopod in order to create a dark, diffuse cloud (much like a ) that can obscure the predator's view, allowing the cephalopod to make a rapid retreat by jetting away.

The second response to a predator is to release pseudomorphs ("false bodies"), smaller clouds of ink with a greater content, which allows them to hold their shape for longer. These are expelled slightly away from the cephalopod in question, which will often release several pseudomorphs and change colour () in conjunction with these releases. The pseudomorphs are roughly the same volume as and look similar to the cephalopod that released them, and many predators have been observed attacking them mistakenly, allowing the cephalopod to escape (this behaviour is often referred to as the "blanch-ink-jet manoeuvre").

Furthermore, ( Chelonia mydas) hatchlings that have been observed mistakenly attacking pseudomorphs released by have subsequently ignored octopuses.


Hiding strategy
The spotty bobtail squid releases ropes of ink longer than itself and hides among them, possibly to be confused with floating leaves.


Behavior around eggs
Octopuses have also been observed squirting ink at or approaching their eggs.

Numerous cuttlefish species add a coat of ink to their eggs, presumably to camouflage them from potential predators.

(2025). 9789251053836, Food and Agriculture Organization.


Use by humans
Cephalopod ink has, as its name suggests, been used in the past as for pens and quills; the name for cuttlefish, and the taxonomic name of a cuttlefish genus, Sepia, is associated with the brown colour of cuttlefish ink (for more information, see sepia).

Modern use of cephalopod ink is generally limited to , primarily in and the Mediterranean, where it is used as a and flavouring, for example in and , and calamares en su tinta. For this purpose, it is generally obtainable from , gourmet food suppliers, and is widely available in markets in Japan,

(2025). 9784431670179, Springer.
and . The ink is extracted from the ink sacs during preparation of the dead cephalopod, usually cuttlefish, and therefore contains no mucus. While it is not commonly used in China, cephalopod ink is sometimes used to dye the dough of .

Studies have shown that cephalopod ink is toxic to some cells, including . It is being researched in mice for its antitumor activity against Meth-A fibrosarcoma. It currently remains unclear however if any of the activity of squid ink can be obtained from oral consumption, and this is indicated as an area for future investigation.


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